dr. A. El Ghalbzouri
Functie:
LUMC
Expertise on modelling healthy and diseased skin
Expertises
- Different human skin equivalents (HSE)available (epidermal model and three full-thickness models)
- Customized assay development for 3Dcultures
- Screening of compounds (topical vs medium supplementation)
- Various 3D-tumor models (e.g., SCC, melanoma, vulvar SCC, head-and neck SCC, breast cancer).
- Tumor microenvironment with different fibroblast subtypes
- Skin aging related studies (epidermal and dermal aging)
- Mimicking diseased skin e.g., models for eczema, psoriasis
- Wound healing: superficial and deep wounds, re-epithelization, scars
- Bacterial colonization/ skin infection
- Effect of external conditions (low oxygen, temp, humidity) on skin and barrier development
Main used techniques
- Tissue engineering
- Cell culture of primary cells
- Gene expression
- Immunohistochemistry
- Luminex (Bio-plex) ELISA
- CRISPRCas9 editing in HSEs
- Imaging